The NEDD8 Monoclonal Antibody (CAB22568) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from mouse cells, is highly specific and reliable for use in various applications such as Western blotting and immunofluorescence.NEDD8 is a small ubiquitin-like modifier that plays a key role in the ubiquitin-proteasome pathway, where it activates and regulates the activity of cullin-RING ligases. Dysregulation of NEDD8 signaling has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEDD8 Monoclonal Antibody
SKU:
CAB22568
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC58460
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NEDD-8, NEDD8
Positive Sample:
293T, RAW264.7, NIH/3T3, C6, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
9kDa
Observed MW:
9kDa
Enables ubiquitin protein ligase binding activity. Acts upstream of or within protein neddylation. Located in cytosol and nucleoplasm. Biomarker of Parkinson's disease and malignant astrocytoma.
Purification Method
Affinity purification
Gene ID
4738
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NEDD8 Rabbit mAb (CAB22568) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
