The NEK11 Polyclonal Antibody (PAC039162) is a valuable tool for researchers studying NEK11, a protein involved in cell cycle regulation and DNA damage response. This antibody, generated in rabbits, exhibits high specificity and sensitivity when used in Western blot applications with human samples. By targeting NEK11, this antibody allows for the detection and analysis of the protein in various cell types, making it a suitable choice for investigations in cancer and cell biology research.NEK11 is a key player in cell division processes, acting as a regulator of mitotic entry and centrosome duplication.
Its involvement in DNA damage repair pathways also highlights its importance in maintaining genomic stability. Research on NEK11 is particularly relevant in the context of cancer, where aberrant cell cycle regulation and DNA repair mechanisms can contribute to tumorigenesis and tumor progression.By utilizing the NEK11 Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of NEK11 in cellular processes, ultimately advancing our understanding of its role in cancer biology and potential therapeutic applications.
Western blot. All lanes: NEK11 antibody at 7µg/ml + Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 75, 55, 56, 69 kDa. Observed band size: 75 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO39162 at dilution of 1:100.
Background:
Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Synonyms:
Serine/threonine-protein kinase Nek11 (EC 2.7.11.1) (Never in mitosis A-related kinase 11) (NimA-related protein kinase 11), NEK11
UniProt Protein Function:
NEK11: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. Interacts with isoform 1 of NEK2. Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2 isoform 1 in G1/S-arrested cells. NEK2 isoform 2 is largely not present in the nucleolus, and does not appear to phosphorylate NEK11. Activated in response to DNA damage. Inhibited by zinc. Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Nucleolus; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; NEK familyChromosomal Location of Human Ortholog: 3q22.1Cellular Component: nucleolus; nucleoplasmMolecular Function: ATP binding; protein binding; protein serine/threonine kinase activityBiological Process: G2/M transition DNA damage checkpoint; histone phosphorylation; intra-S DNA damage checkpoint; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]
