The NEK6 Monoclonal Antibody (CAB3536) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and is suitable for use in Western blotting and immunohistochemistry applications. By binding to NEK6, this antibody enables the detection and analysis of the protein in various cell types, making it useful for studies in cell biology and cancer research.NEK6 is known to play a critical role in cell division and the maintenance of genomic stability.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEK6 Monoclonal Antibody
SKU:
CAB3536
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2034
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
ETTA AHSL VGTP YYMS PERI HENG YNFK SDIW SLGC LLYE MAAL QSPF YGDK MNLF SLCQ KIEQ CDYP PLPG EHYS EKLR ELVS MCIC PDPH QRPD IGYV H
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SID6-1512, NEK6
Positive Sample:
293T, K-562, Mouse lung, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Nucleus Speckle, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole.
Calculated MW:
36kDa
Observed MW:
35kDa
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10783
RRID
AB_2863083
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NEK6 Rabbit mAb (CAB3536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Western blot analysis of various lysates using NEK6 Rabbit mAb (CAB3536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
