The NFASC Antibody (CAB3053) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in various applications, including Western blotting.Neurofascin is a key player in the organization of neuronal networks and the proper functioning of nerve cells. Dysregulation of neurofascin expression has been implicated in disorders affecting the nervous system, such as multiple sclerosis and autism spectrum disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NFASC Antibody
SKU:
CAB3053
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NF, NRCAML, NEDCPMD, NFASC
Positive Sample:
SH-SY5Y, HeLa, HL-60, Mouse brain, Mouse pancreas, Rat brain
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
150kDa
Observed MW:
150kDa
This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.
Purification Method
Affinity purification
Gene ID
23114
RRID
AB_2764857
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NFASC Rabbit pAb (CAB3053) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
