The NEXN Antibody (CAB13136) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the NEXN protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an essential tool for investigations in cell biology and cancer research.NEXN, also known as Nexilin, plays a pivotal role in maintaining cell structure and function, making it a key target for understanding processes like cell motility and tissue development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEXN Antibody
SKU:
CAB13136
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CMH20, NELIN, NEXN
Positive Sample:
SGC-7901, Mouse testis, Rat brain, Rat spleen, Rat testis
Cellular Localization:
Cell Junction, Cytoplasm, Z Line, Adherens Junction, Cytoskeleton, Myofibril, Sarcomere.
Calculated MW:
81kDa
Observed MW:
81kDa
This gene encodes a filamentous actin-binding protein that may function in cell adhesion and migration. Mutations in this gene have been associated with dilated cardiomyopathy, also known as CMD1CC. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
91624
RRID
AB_2759986
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NEXN Rabbit pAb (CAB13136) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using NEXN Rabbit pAb (CAB13136) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
