The NIP7 Antibody (CAB4734) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit serum, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the NIP7 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in molecular biology and cancer research.NIP7 is a key player in the intricate process of ribosome assembly, essential for protein synthesis in cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
NIP7 Antibody
SKU:
CAB4734
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KD93, CGI-37, HSPC031, NIP7
Positive Sample:
HL-60, HT-1080, SW620, Hela, A375
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
20kDa
Observed MW:
20kDa
Enables RNA binding activity. Predicted to be involved in ribosomal large subunit biogenesis. Located in cytosol; nucleolus; and nucleoplasm.
Purification Method
Affinity purification
Gene ID
51388
RRID
AB_2765840
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NIP7 Rabbit pAb (CAB4734) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
