The NKX3-2 Antibody (CAB8093) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it a reliable choice for Western blot analysis.Nkx3.2, also referred to as Bapx1, plays a crucial role in regulating gene expression and controlling cell differentiation during embryonic development. Its expression has been linked to various musculoskeletal disorders, making it a potential target for therapeutic interventions aimed at treating conditions such as osteoarthritis and chondrodysplasia.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NKX3-2 Antibody
SKU:
CAB8093
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SMMD, BAPX1, NKX3B, NKX3.2, NKX3-2
Positive Sample:
293T transfected with NKX3-2, Mouse spleen
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
40kDa/46kDa
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in cell differentiation; negative regulation of chondrocyte differentiation; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including animal organ development; embryonic skeletal system development; and intestinal epithelial cell development. Predicted to be part of chromatin. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
579
RRID
AB_2770594
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NKX3-2, using NKX3-2 Rabbit pAb (CAB8093) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse spleen, using NKX3-2 Rabbit pAb (CAB8093) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
