The NLRC4 Polyclonal Antibody (CAB24336) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been shown to have high reactivity with human samples and is suitable for Western blot applications. By binding specifically to the NLRC4 protein, this antibody allows for the detection and analysis of NLRC4 in various cell types, making it ideal for studies in immunology and infectious disease research.NLRC4, also known as Nod-like receptor C4, is an important regulator of inflammation and plays a crucial role in the response to bacterial and viral infections.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NLRC4 Polyclonal Antibody
SKU:
CAB24336
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
268973
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse large intestine, using NLRC4 Rabbit pAb (CAB24336) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded human spleen using NLRC4 Rabbit pAb (CAB24336) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of PC-12 cells using NLRC4 Rabbit pAb (CAB24336) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
