Description
NLRP3 Antibody (CAB12694)
The NLRP3 Antibody (CAB12694) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reacts with human samples, making it ideal for Western blot applications. By binding to the NLRP3 protein, it allows for precise detection and analysis in various cell types, opening up possibilities for research in immunology and inflammatory diseases.NLRP3, also known as the NLR family pyrin domain-containing 3, is a crucial component of the inflammasome complex, which activates the inflammatory response in the presence of danger signals.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | NLRP3 Antibody |
| SKU: | CAB12694 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MKMA STRC KLAR YLED LEDV DLKK FKMH LEDY PPQK GCIP LPRG QTEK ADHV DLAT LMID FNGE EKAW AMAV WIFA AINR RDLY EKAK RDEP KWGS DNAR VSNP TVIC QEDS IEEE WMGL LEYL SRIS ICKM KKDY RKKY RKYV RSRF QCIE DRNA RLGE SVSL NKRY TRLR LIKE HRSQ QERE QELL AIGK TKTC ESPV SPIK MELL FDPD DEHS EPVH | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | AII, AVP, FCU, MWS, FCAS, KEFH, CIAS1, FCAS1, NALP3, C1orf7, CLR1.1, DFNA34, PYPAF1, AGTAVPRL, NLRP3 |
| Cellular Localization: | Cytoplasm, Endoplasmic Reticulum, Inflammasome, Nucleus, Secreted, Cytosol. |
| Calculated MW: | 118kDa |
| Observed MW: | Refertofigures |
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
| Purification Method | Affinity purification |
| Gene ID | 114548 |
| RRID | AB_2759538 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using NLRP3 Rabbit pAb (CAB12694) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Jurkat. Exposure time: 10s. |
 | Immunofluorescence analysis of RAW264.7 cells using NLRP3 Rabbit pAb (CAB12694) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
| Mitchell, D.K., et al. | Inhibition of focal adhesion kinase impairs tumor formation and preserves hearing in a murine model of NF2-related schwannomatosis | Oncology 2026 | PubMed ID: 41616055 |
