Description
NLRP3 Antibody (CAB5652)
The NLRP3 Antibody (CAB5652) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to human samples, making it ideal for use in Western blot applications. By targeting the NLRP3 protein, researchers can gain insights into its role in various cell types and its implications for diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | NLRP3 Antibody |
| SKU: | CAB5652 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MTSV RCKL AQYL EDLE DVDL KKFK MHLE DYPP EKGC IPVP RGQM EKAD HLDL ATLM IDFN GEEK AWAM AVWI FAAI NRRD LWEK AKKD QPEW N | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | AII, AVP, FCU, MWS, FCAS, KEFH, CIAS1, FCAS1, NALP3, C1orf7, CLR1.1, DFNA34, PYPAF1, AGTAVPRL, NLRP3 |
| Positive Sample: | RAW 264.7 treated with LPS |
| Cellular Localization: | Cytoplasm, Endoplasmic Reticulum, Inflammasome, Nucleus, Secreted, Cytosol. |
| Calculated MW: | 118kDa |
| Observed MW: | 118kDa |
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
| Purification Method | Affinity purification |
| Gene ID | 114548 |
| RRID | AB_2766412 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from RAW 264.7 cells using NLRP3 Rabbit pAb (CAB5652) at 1:1800 dilution incubated overnight at 4℃. Raw264.7 cells were treated with LPS (10 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HepG2 cells using NLRP3 Rabbit pAb (CAB5652) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
