The NOD1 Polyclonal Antibody (CAB1246) is a valuable tool for research involving NOD1, a cytosolic pattern recognition receptor involved in innate immune responses. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the NOD1 protein, this antibody enables the detection and analysis of NOD1 in various cell types, making it an essential tool for studies in immunology and infectious disease research.NOD1 is a critical player in the innate immune system, specifically recognizing bacterial peptidoglycans and initiating signaling cascades that lead to the production of pro-inflammatory cytokines.
Understanding the role of NOD1 in immunity is crucial for developing strategies to combat bacterial infections and inflammatory diseases. Research using the NOD1 Polyclonal Antibody can provide valuable insights into the mechanisms of innate immune responses and help identify potential therapeutic targets in infectious and inflammatory conditions.
Product Name:
NOD1 Rabbit Polyclonal Antibody
SKU:
CAB1246
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NOD1 (NP_006083.1).
This gene encodes a member of the nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins. The encoded protein plays a role in innate immunity by acting as a pattern-recognition receptor (PRR) that binds bacterial peptidoglycans and initiates inflammation. This protein has also been implicated in the immune response to viral and parasitic infection. Major structural features of this protein include an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. Mutations in this gene are associated with asthma, inflammatory bowel disease, Behcet disease and sarcoidosis in human patients.
Purification Method:
Affinity purification
Gene ID:
10392
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NOD1 Rabbit pAb (CAB1246) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
