The NOL12 Antibody (CAB14292) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables identical protein binding activity. Predicted to be active in nucleolus.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NOL12 Antibody
SKU:
CAB14292
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Nop25, dJ37E16.7, NOL12
Positive Sample:
Raji, A-431, HeLa, HepG2, SH-SY5Y
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
25kDa
Observed MW:
36kDa
Enables identical protein binding activity. Predicted to be active in nucleolus.
Purification Method
Affinity purification
Gene ID
79159
RRID
AB_2761154
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NOL12 Rabbit pAb (CAB14292) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
