The NOP14 Antibody (CAB10361) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot analysis. By binding specifically to the NOP14 protein, this antibody enables precise detection and analysis in different cell types, making it ideal for research in molecular biology and cancer biology.NOP14 is a key player in ribosome assembly, a fundamental process essential for protein synthesis in cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NOP14 Antibody
SKU:
CAB10361
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UTP2, NOL14, C4orf9, RES425, RES4-25, NOP14
Positive Sample:
HeLa, U-87MG, HT-29, Jurkat, MCF7
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
98kDa
Observed MW:
115kDa
This gene encodes a protein that plays a role in pre-18s rRNA processing and small ribosomal subunit assembly. The encoded protein may be involved in the regulation of pancreatic cancer cell proliferation and migration. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8602
RRID
AB_2757907
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOP14 Rabbit pAb (CAB10361) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
