The NPM1 Polyclonal Antibody (CAB17983) is a valuable tool for researchers studying NPM1, a nucleolar phosphoprotein involved in ribosome biogenesis and regulation of cell proliferation. This rabbit-derived antibody is highly specific for human samples and has been validated for use in Western blot applications. By targeting the NPM1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cancer biology and cellular physiology.NPM1 is known for its role in maintaining genomic stability and promoting cell growth, making it a key player in tumorigenesis and cancer progression.
By understanding the function and regulation of NPM1, researchers can gain valuable insights into the mechanisms underlying cancer development and potentially identify novel therapeutic targets for cancer treatment. The NPM1 Polyclonal Antibody is a valuable tool for researchers looking to delve deeper into the molecular pathways involving NPM1 and its implications in cancer biology.
Product Name:
NPM1 Rabbit Polyclonal Antibody
SKU:
CAB17983
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 9-122 of human NPM1 (NP_002511.1).
Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, nucleoplasm.
Calculated MW:
33kDa
Observed MW:
38kDa
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified.
Purification Method:
Affinity purification
Gene ID:
4869
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using NPM1 Rabbit pAb (CAB17983) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
