The NSDHL Monoclonal Antibody is a valuable tool for research involving the NSDHL protein, a crucial enzyme in the cholesterol biosynthesis pathway. This antibody, developed using advanced monoclonal technology, is highly specific to human NSDHL and is validated for use in various applications such as immunofluorescence and immunohistochemistry.NSDHL, also known as NAD(P)H steroid dehydrogenase-like protein, plays a vital role in the biosynthesis of cholesterol, a key component of cell membranes and a precursor to important signaling molecules.
Dysregulation of NSDHL activity has been implicated in various diseases, including Smith-Lemli-Opitz syndrome and certain types of cancer.By using the NSDHL Monoclonal Antibody, researchers can accurately detect and analyze NSDHL expression in different cell types and tissues, providing valuable insights into its functions and potential therapeutic targets. This antibody is a valuable tool for studies in molecular biology, biochemistry, and lipid metabolism, leading to a better understanding of cholesterol homeostasis and related disorders.
Product Name:
NSDHL Monoclonal Antibody
SKU:
CAB21152
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human NSDHL (NP_057006.1).
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
50814
Clone Number:
ARC3040
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using NSDHL Rabbit mAb (CAB21152) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
