The NT5C1A Polyclonal Antibody (PACO23425) is specifically designed for research involving NT5C1A, a nucleotidase enzyme involved in nucleotide metabolism. This antibody, derived from rabbit immunization, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the NT5C1A protein, researchers can effectively detect and analyze this enzyme in various cell types, making it an ideal tool for studies in biochemistry and molecular biology.NT5C1A plays a crucial role in maintaining cellular nucleotide balance and regulating purine metabolism.
Dysregulation of NT5C1A has been linked to various diseases, including cancer and metabolic disorders. By understanding the function and expression of NT5C1A, researchers can gain insights into the mechanisms underlying these diseases and potentially develop targeted therapies.Overall, the NT5C1A Polyclonal Antibody serves as a valuable tool for researchers studying nucleotide metabolism, purine regulation, and related diseases. Its high reactivity and specificity make it a reliable choice for experiments aimed at elucidating the role of NT5C1A in various cellular processes.
Synthesized peptide derived from internal of human NT5C1A.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from MCF-7 cells and HepG2 cells, using NT5C1A antibody.
Immunofluorescence analysis of A549 cells, using NT5C1A antibody.
Background:
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.
Synonyms:
nucleotidase; cytosolic IA; 5NT1A; CN-I; CN-IA
UniProt Protein Function:
NT5C1A: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia. Belongs to the 5'-nucleotidase type 3 family.Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Phosphatase (non-protein)Chromosomal Location of Human Ortholog: 1p34.3-p33Cellular Component: cytosolMolecular Function: 5'-nucleotidase activity; magnesium ion binding; nucleotide bindingBiological Process: adenosine metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; nucleoside metabolic process; purine base metabolic process; purine nucleoside monophosphate catabolic process; purine nucleotide catabolic process; pyrimidine base metabolic process; pyrimidine nucleoside catabolic process
UniProt Protein Details:
NCBI Summary:
Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008]
