The NTAN1 Antibody (CAB15562) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in a variety of applications, including Western blotting. By targeting the NTAN1 protein, this antibody allows for the detection and analysis of NTAN1 levels in different cell types, making it ideal for studies in biochemistry and cellular biology.NTAN1 is a key player in the N-terminal acetylation process, which is essential for the regulation of protein stability, localization, and function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NTAN1 Antibody
SKU:
CAB15562
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PNAA, PNAD, NTAN1
Positive Sample:
293T, Raji, HepG2, MCF-7, Mouse spleen, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
35kDa
Observed MW:
35kDa
The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Purification Method
Affinity purification
Gene ID
123803
RRID
AB_2762966
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NTAN1 Rabbit pAb (CAB15562) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
