The NTF4 Antibody (CAB1081) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in various applications, including Western blot and immunohistochemistry.Neurotrophin-4 is known for its neurotrophic and neuroprotective properties, making it a key player in neuronal survival, differentiation, and synaptic plasticity. By targeting neurotrophin-4 with this antibody, researchers can study its expression and localization in different cell types and tissues, shedding light on its role in neurodevelopment, neurodegenerative disorders, and neurological diseases.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NTF4 Antibody
SKU:
CAB1081
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
QPPP STLP PFLA PEWD LLSP RVVL SRGA PAGP PLLF LLEA GAFR ESAG APAN RSRR GVSE TAPA SRRG ELAV CDAV SGWV TDRR TAVD LRGR EVEV LGEV PAAG GSPL RQYF FETR CKAD NAEE GGPG AGGG GCRG VDRR HWVS ECKA KQSY VRAL TADA QGRV GWRW IRID TACV CTLL SRTG RA
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:100 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
Purification Method
Affinity purification
Gene ID
4909
RRID
AB_2758238
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NTF4 Rabbit pAb (CAB1081) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human liver damage using NTF4 Rabbit pAb (CAB1081) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of A-549 cells using NTF4 Rabbit pAb (CAB1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using NTF4 Rabbit pAb (CAB1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
