The NTPCR Antibody (CAB14944) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been specifically validated for use in Western blot applications with human samples. By targeting the NTPCR protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology, endocrinology, and metabolism research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
NTPCR Antibody
SKU:
CAB14944
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
THEP1, C1orf57, HCR-NTPase, NTPCR
Positive Sample:
U-251MG, A-431, 293T
Calculated MW:
21kDa
Observed MW:
21kDa
The protein encoded by this gene is a non-specific nucleoside triphosphatase that is slow-acting in vitro. This gene is overexpressed in many tumor tissues, and while it is not essential for the cell, overexpression is cytotoxic. However, the cytotoxicity is not related to its triphosphatase activity.
Purification Method
Affinity purification
Gene ID
84284
RRID
AB_2761826
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NTPCR Rabbit pAb (CAB14944) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using NTPCR Rabbit pAb (CAB14944) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
