Nuclear Matrix Protein p84 (THOC1) Monoclonal Antibody (CAB9269)
The Nuclear Matrix Protein p84 (THOC1) Monoclonal Antibody (CAB9269) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable DNA binding activity and RNA binding activity. Involved in several processes, including negative regulation of DNA damage checkpoint; regulation of nucleobase-containing compound metabolic process; and viral mRNA export from host cell nucleus. Located in cytoplasm and nuclear speck. Part of THO complex part of transcription export complex. Colocalizes with chromosome, telomeric region.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Nuclear Matrix Protein p84 (THOC1) Monoclonal Antibody
SKU:
CAB9269
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1504
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P84, HPR1, P84N5, DFNA86, Nuclear Matrix Protein p84 (THOC1)
Predicted to enable DNA binding activity and RNA binding activity. Involved in several processes, including negative regulation of DNA damage checkpoint; regulation of nucleobase-containing compound metabolic process; and viral mRNA export from host cell nucleus. Located in cytoplasm and nuclear speck. Part of THO complex part of transcription export complex. Colocalizes with chromosome, telomeric region.
Purification Method
Affinity purification
Gene ID
9984
RRID
AB_2863702
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Nuclear Matrix Protein p84 (THOC1) (THOC1) Rabbit mAb (CAB9269) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation of Nuclear Matrix Protein p84 (THOC1) from 300 µg extracts of 293F cells was performed using 3 µg of Nuclear Matrix Protein p84 (THOC1) Rabbit mAb (CAB9269). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Nuclear Matrix Protein p84 (THOC1) Rabbit mAb (CAB9269) at a dilution of 1:1000.
