The NXT2 Antibody (CAB13822) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to NXT2, this antibody allows for the detection and analysis of the protein in different cell types, making it ideal for studies in cell biology and molecular biology.NXT2 is known to play a role in RNA processing and transport within the cell, making it a key player in gene expression regulation. Understanding the function of NXT2 is crucial for unraveling the complexities of cellular mechanisms, particularly in the context of gene regulation and protein synthesis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NXT2 Antibody
SKU:
CAB13822
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P15-2, NXT2
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
16kDa
Observed MW:
16kDa
The protein encoded by this gene contains a nuclear transport factor 2 (NTF2) domain, which plays an important role in the trafficking of macromolecules, ions, and small molecules between the cytoplasm and nucleus. This protein may also have a role in mRNA nuclear export. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
55916
RRID
AB_2760676
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NXT2 Rabbit pAb (CAB13822) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using NXT2 Rabbit pAb (CAB13822) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
