The OPA3 Antibody (CAB7997) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for OPA3 in human samples and has been validated for use in Western blot applications. By binding to OPA3, the antibody enables researchers to detect and analyze the protein in various cell types, making it ideal for studies in mitochondrial biology and related diseases.OPA3 plays a critical role in maintaining mitochondrial morphology and function, making it a target of interest in research on mitochondrial dynamics and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
OPA3 Antibody
SKU:
CAB7997
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MGA3, OPA3
Positive Sample:
Mouse eye, Rat eye
Cellular Localization:
Mitochondrion.
Calculated MW:
20kDa
Observed MW:
20kDa
The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
80207
RRID
AB_2770724
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using OPA3 Rabbit pAb (CAB7997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
