The ORC1 Antibody (CAB14756) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blotting applications. By binding to the ORC1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.ORC1, also known as Origin Recognition Complex subunit 1, plays a crucial role in initiating DNA replication and ensuring genomic stability. Dysregulation of ORC1 has been linked to various human cancers and genetic disorders, highlighting its importance in understanding disease mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ORC1 Antibody
SKU:
CAB14756
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ORC1L, PARC1, HSORC1, ORC1
Positive Sample:
HeLa, 293T, A-431, K-562, Jurkat, MCF7, A-549
Cellular Localization:
Nucleus.
Calculated MW:
97kDa
Observed MW:
90kDa
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4998
RRID
AB_2761632
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ORC1 Rabbit pAb (CAB14756) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
