The ORC3 Antibody (CAB15415) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot experiments. By binding specifically to the ORC3 protein, this antibody allows for precise and accurate detection and analysis in a variety of cell types.ORC3, part of the Origin Recognition Complex, is essential for the initiation of DNA replication and the maintenance of genomic stability. Dysregulation of ORC3 has been linked to various diseases, including cancer, developmental disorders, and neurodegenerative conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ORC3 Antibody
SKU:
CAB15415
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LAT, ORC3L, LATHEO, ORC3
Positive Sample:
LO2, 293T, HeLa, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
82kDa
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene.
Purification Method
Affinity purification
Gene ID
23595
RRID
AB_2762323
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ORC3 Rabbit pAb (CAB15415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
