The OTX2 Monoclonal Antibody (CAB4351) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, produced in rabbits, exhibits exceptional reactivity with human samples and has been rigorously validated for use in applications such as Western blotting and immunofluorescence.OTX2 is a key regulator of neural development, controlling processes such as neuronal differentiation and patterning in the brain. Dysregulation of OTX2 has been linked to various neurological disorders and brain tumors, emphasizing the importance of studying its function in both normal and pathological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
OTX2 Monoclonal Antibody
SKU:
CAB4351
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0973
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CPHD6, MCOPS5, OTX2
Positive Sample:
Mouse eye, Rat eye
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
32kDa
This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine.
Purification Method
Affinity purification
Gene ID
5015
RRID
AB_2863244
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using OTX2 Rabbit mAb (CAB4351) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
