The OXCT2 Antibody (CAB14920) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry.OXCT2 is a key enzyme in the ketogenesis pathway, responsible for converting acetoacetyl-CoA to acetoacetate. Dysregulation of ketone body metabolism has been implicated in various metabolic disorders, making OXCT2 an important target for research in the fields of metabolism and energy homeostasis.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
OXCT2 Antibody
SKU:
CAB14920
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SCOTT, FKSG25, OXCT2
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Mitochondrion.
Calculated MW:
56kDa
Observed MW:
55kDa
The protein encoded by this gene catalyzes the transfer of a CoA group from succinate to acetoacetate and is an important enzyme in ketone body catabolism. The encoded protein localizes to the mitochondrion. This gene is intronless, and a pseudogene of this gene is located elsewhere on chromosome 1.
Purification Method
Affinity purification
Gene ID
64064
RRID
AB_2761802
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using OXCT2 Rabbit pAb (CAB14920) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of paraffin-embedded rat testis using OXCT2 Rabbit pAb (CAB14920) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
