The OxER1 Antibody (PAC055222) is a polyclonal antibody developed for research on the OxER1 protein, a key regulator of oxysterol-induced inflammatory response in cells. This antibody, produced in rabbits, has high specificity and sensitivity for detecting OxER1 in human samples, making it an essential tool for studies in the fields of lipid metabolism, inflammation, and cardiovascular diseases.OxER1, also known as GPR183, plays a crucial role in mediating the cellular response to oxysterols, which are oxidized derivatives of cholesterol involved in inflammatory signaling pathways. By targeting OxER1, researchers can gain insights into the mechanisms underlying oxysterol-induced inflammation and potentially identify new therapeutic targets for conditions such as atherosclerosis, autoimmune diseases, and neurodegenerative disorders.
With its ability to detect OxER1 in various cell types and tissues, the OxER1 Antibody is suitable for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. By studying the expression and function of OxER1, researchers can advance our understanding of the role of oxysterols in health and disease, paving the way for the development of novel treatments and interventions.
Western Blot. Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Rat lung tissue. All lanes: OXER1 antibody at 2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.
Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO55222 at dilution of 1:100.
Background:
Receptor for eicosanoids and polyunsaturated fatty acid, such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid, (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid, (5(S)-HPETE) and arachidonic acid, Seems to be coupled to the G(i)/G(o), families of heteromeric G proteins.
OXER1: Receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. Seems to be coupled to the G(i)/G(o), families of heteromeric G proteins. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1Chromosomal Location of Human Ortholog: 2p21Cellular Component: integral to membrane; plasma membraneMolecular Function: 5(S)-hydroxyperoxy-6E,8Z,11Z,14Z-icosatetraenoic acid binding; 5-hydroxy-6E,8Z,11Z,14Z-icosatetraenoic acid binding; 5-oxo-6E,8Z,11Z,14Z-icosatetraenoic acid binding; G-protein coupled receptor activityBiological Process: G-protein coupled receptor protein signaling pathway; regulation of cAMP biosynthetic process
