The PAI-1/Serpin E1 Monoclonal Antibody (CAB19096) is a high-quality antibody developed for reliable detection and analysis of target proteins. PAI1 is a key regulator of the fibrinolytic system and plays a crucial role in controlling blood clot formation and degradation. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for a wide range of research applications. It has been validated for use in Western blot and immunohistochemistry, allowing for the detection and analysis of PAI1 expression in various cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Monkey samples.
Product Name:
PAI-1/Serpin E1 Monoclonal Antibody
SKU:
CAB19096
Size:
20μL, 100μL
Reactivity:
Human, Monkey
Clone Number:
ARC0473
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAI, PAI1, PAI-1, PLANH1, PAI-1/Serpin E1
Positive Sample:
HuH-7, U-2 OS, COS-7
Cellular Localization:
Secreted.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.
Purification Method
Affinity purification
Gene ID
5054
RRID
AB_2862588
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PAI-1/Serpin E1 Rabbit mAb (CAB19096) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
