The pan-Mono-Methyl Arginine Motif Antibody (CAB17984) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for mono-methyl arginine motifs and has been validated for use in Western blot applications. It binds specifically to mono-methyl arginine residues, enabling accurate detection and analysis of these modifications in a variety of cell types.Protein methylation, including mono-methylation of arginine residues, plays a crucial role in regulating protein function, cellular signaling, and gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
pan-Mono-Methyl Arginine Motif Antibody
SKU:
CAB17984
Size:
100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, HCT116, MCF7, Mouse brain, Rat brain
Observed MW:
10-130kDa
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using pan-Mono-Methyl R Motif Rabbit pAb (CAB17984) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
