The PAPSS1 Antibody (CAB13773) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.PAPSS1 plays a crucial role in sulfation reactions, which are vital for the structure and function of various molecules in the body. Dysregulation of PAPSS1 activity has been implicated in diseases such as cancer, cardiovascular disorders, and metabolic syndromes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PAPSS1 Antibody
SKU:
CAB13773
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
SEYE KPEA PELV LKTD SCDV NDCV QQVV ELLQ ERDI VPVD ASYE VKEL YVPE NKLH LAKT DAET LPAL KIN
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SK1, PAPSS, ATPSK1, PAPSS1
Positive Sample:
LO2, HT-29, 293T, Mouse brain, Rat kidney
Cellular Localization:
Cytosol.
Calculated MW:
71kDa
Observed MW:
71kDa
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).
Purification Method
Affinity purification
Gene ID
9061
RRID
AB_2760630
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PAPSS1 Rabbit pAb (CAB13773) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
