The PARP10 Antibody (CAB12815) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PARP10 protein, this antibody enables detection and analysis in a variety of cell types, making it suitable for studies in molecular biology, genetics, and cancer research.PARP10, also known as ARTD10, is a key player in maintaining genomic stability and DNA repair processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PARP10 Antibody
SKU:
CAB12815
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARTD10, PARP10
Positive Sample:
HepG2, 293T, HT-29, MCF7, HeLa
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
110kDa
Observed MW:
110kDa
Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 [PubMed 15674325]).
Purification Method
Affinity purification
Gene ID
84875
RRID
AB_2759655
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PARP10 Rabbit pAb (CAB12815) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
