The PCF11 Antibody (CAB16533) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.PCF11 is known to play a crucial role in regulating gene expression and RNA splicing, making it a key player in maintaining cellular homeostasis. Its dysregulation has been linked to diseases such as cancer, neurological disorders, and developmental abnormalities, making it an attractive target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PCF11 Antibody
SKU:
CAB16533
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCF11
Positive Sample:
LO2, Jurkat, HeLa, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
173kDa
Observed MW:
180kDa
The protein encoded by this gene binds to CLP1 to form pre-mRNA cleavage factor IIm. The encoded protein is necessary for efficient Pol II transcription termination and may be involved in degradation of the 3' product of polyA site cleavage.
Purification Method
Affinity purification
Gene ID
51585
RRID
AB_2770810
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PCF11 Rabbit pAb (CAB16533) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
