The ALIX/PDCD6IP Antibody (CAB2215) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to PDCD6IP, researchers can effectively detect and analyze this protein in various cell types, facilitating studies in cell biology, apoptosis, and calcium signaling pathways.PDCD6IP, also called Programmed cell death 6 Interacting Protein, is involved in various cellular processes, including cell death, cell cycle regulation, and intracellular signaling.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ALIX/PDCD6IP Antibody
SKU:
CAB2215
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
Purification Method
Affinity purification
Gene ID
10015
RRID
AB_2764230
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ALIX / PDCD6IP Rabbit pAb (CAB2215) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunofluorescence analysis of L929 cells using ALIX / PDCD6IP Rabbit pAb (CAB2215) at dilution of 1:100. Blue: DAPI for nuclear staining.
