The PDE12 Antibody (CAB17270) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables 3'-5'-exoribonuclease activity. Involved in several processes, including RNA metabolic process; cellular response to cytokine stimulus; and regulation of mitochondrial mRNA stability. Located in mitochondrial matrix. RRID AB_2770823 Gene ID 201626 Swiss Prot Synonym 2-PDE; 2'-PDE; PDE12
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PDE12 Antibody
SKU:
CAB17270
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
-
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
2-PDE, 2'-PDE, PDE12
Positive Sample:
Mouse thymus, Rat thymus
Cellular Localization:
Cytosol, Mitochondrial Matrix, Mitochondrion.
Calculated MW:
67kDa
Observed MW:
67kDa
Enables 3'-5'-exoribonuclease activity. Involved in several processes, including RNA metabolic process; cellular response to cytokine stimulus; and regulation of mitochondrial mRNA stability. Located in mitochondrial matrix. RRID AB_2770823 Gene ID 201626 Swiss Prot Synonym 2-PDE; 2'-PDE; PDE12
Purification Method:
Affinity purification
Gene ID:
201626
RRID:
AB_2770823
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDE12 Rabbit pAb (CAB17270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using PDE12 Rabbit pAb (CAB17270) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
