The PDLIM2 Polyclonal Antibody (PAC016850) is a valuable tool for researchers studying PDLIM2, a protein involved in cell signaling and regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the PDLIM2 protein, allowing for precise detection and analysis in various cell types. PDLIM2, also known as PDZ and LIM domain protein 2, plays a crucial role in diverse cellular processes such as cytoskeletal organization, cell adhesion, and gene transcription.
Its involvement in these pathways makes it a promising target for research in fields like cell biology, cancer biology, and developmental biology. By studying the function of PDLIM2, researchers can gain insights into its role in disease development and potentially identify novel therapeutic strategies. Overall, the PDLIM2 Polyclonal Antibody is a valuable tool for investigating the functions and mechanisms of PDLIM2 in various biological contexts, providing researchers with the means to further our understanding of this important protein.
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue, Primary antibody: PACO16850(PDLIM2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO16850(PDLIM2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Synonyms:
PDZ and LIM domain 2 (mystique)
UniProt Protein Function:
PDLIM2: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; Cell adhesionChromosomal Location of Human Ortholog: 8p21.3Cellular Component: cortical actin cytoskeleton; focal adhesion; stress fiber; nucleus; actin cytoskeletonMolecular Function: zinc ion binding; filamin binding; muscle alpha-actinin binding; myosin heavy chain binding
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
