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PE Anti-Mouse CD34 Antibody [RAM34] (AGEL3004)

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SKU AGEL3004
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FCM
Reactivity Mouse
Host Species Rat
Isotype Rat IgG2a, κ
Clone RAM34
Conjugation PE
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Description

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PE Anti-Mouse CD34 Antibody [RAM34]

The PE Anti-Mouse CD34 Antibody (RAM34-AGEL3004) is a valuable tool for researchers studying CD34, a transmembrane glycoprotein involved in cell adhesion and migration. This antibody, specifically designed for use in flow cytometry, is well-suited for identifying and quantifying mouse CD34 expression in various cell types.CD34 is a marker of hematopoietic stem and progenitor cells, as well as vascular endothelial progenitor cells, making it a key player in hematopoiesis and angiogenesis. The PE Anti-Mouse CD34 Antibody binds specifically to CD34, allowing for precise detection and analysis of this important marker in experimental settings.

By utilizing the PE Anti-Mouse CD34 Antibody, researchers can gain insights into the role of CD34 in processes such as stem cell differentiation, vascular development, and tissue regeneration. This antibody is a valuable resource for studies focusing on hematopoiesis, vasculogenesis, and the development of targeted therapies for various diseases.Overall, the PE Anti-Mouse CD34 Antibody is a reliable tool that offers high specificity and sensitivity, making it an essential component for investigations into the function and expression of CD34 in biological systems.

Product Title:PE Anti-Mouse CD34 Antibody [RAM34]
SKU:AGEL3004
Size:50 Tests, 100 Tests, 200 Tests
Clone No:RAM34
Synonyms:Hematopoietic progenitor cell antigen CD34;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2a, κ
Isotype Control:PE Rat IgG2a, κ Isotype Control[2A3]
Form:Liquid
Conjugation:PE
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:Q64314
Gene ID:12490
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.

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