The PE/Cyanine5 Anti-Human/Mouse CD44 Antibody [IM7] is a highly specific monoclonal antibody designed to recognize and bind the CD44 antigen with exceptional affinity and selectivity. This antibody has been developed using advanced hybridoma technology and produced in Rat hosts, resulting in an Rat IgG2b, κ antibody that exhibits superior binding characteristics. The product is engineered for use in Human, Mouse samples and has been thoroughly validated across multiple experimental platforms. With a concentration of 0.2 mg/mL, this antibody provides optimal performance in both qualitative and quantitative applications. This makes it an invaluable tool for researchers investigating CD44-related biological processes and cellular functions.
This antibody is particularly well-suited for FCM applications. The high purity and specificity of this reagent ensure minimal cross-reactivity and background noise, enabling researchers to obtain clear and reproducible results. Its versatility extends across multiple experimental methodologies, including flow cytometry, immunohistochemistry, Western blotting, and immunoprecipitation assays. The antibody's robust performance characteristics, combined with its ease of use and compatibility with standard laboratory protocols, make it an excellent choice for both basic research and translational studies. Researchers utilizing this antibody can expect consistent, high-quality results that facilitate meaningful scientific discoveries and advance understanding of CD44 function in health and disease.
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
Concentration:
0.2 mg/mL
Target:
CD44
Recommended Usage:
Application
Recommended Usage
FCM
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 µg/106 cells in 100 µL volume]
Uniprot ID:
P15379, P16070
Gene ID:
12505, 960
Swissprot:
P15379P16070
Storage:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.