The PE GenieFluor 594 Anti-Mouse F4/80 Antibody (CI:A3-1) is a cutting-edge research tool designed for immunofluorescence studies involving mouse F4/80, a widely used marker for macrophages and monocytes. This antibody, specifically targeting the F4/80 protein, is produced for use in flow cytometry applications and is validated for its high specificity and sensitivity.F4/80 is a well-established cell surface marker that plays a crucial role in identifying and characterizing macrophages in various tissues. The precise binding of the PE GenieFluor 594 Anti-Mouse F4/80 Antibody allows for the accurate detection and visualization of F4/80-positive cells in immunofluorescence experiments.
Its compatibility with flow cytometry techniques makes it a versatile tool for researchers studying immune responses, inflammation, and tissue macrophage biology in mouse models.By targeting F4/80, researchers can gain valuable insights into the function and behavior of macrophages in different biological contexts, including immune regulation, host defense, and inflammation. The PE GenieFluor 594 Anti-Mouse F4/80 Antibody is an essential resource for advancing our understanding of macrophage biology and its implications in various disease processes.
PE/Genie Fluor 594 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
PE/Genie Fluor594
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
Q61549
Gene ID:
13733
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
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