The PELP1 Monoclonal Antibody (CAB9026) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.PELP1, also known as proline, glutamic acid, and leucine-rich protein 1, plays a significant role in hormone signaling pathways and has been linked to hormone-dependent cancers such as breast and prostate cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PELP1 Monoclonal Antibody
SKU:
CAB9026
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1387
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
EPEP GLLL EVEE PGTE EERG ADTA PTLA PEAL PSQG EVER EGES PAAG PPPQ ELVE EEPS APPT LLEE ETED GSDK VQPP PETP AEEE METE TEAE ALQE K
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MNAR, P160, PELP1
Positive Sample:
SW620, MCF7, SKOV3, NIH/3T3, Rat testis
Cellular Localization:
Cytoplasm, Nucleus, Nucleoplasm.
Calculated MW:
120kDa
Observed MW:
160kDa
This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
27043
RRID
AB_2863644
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PELP1 Rabbit mAb (CAB9026) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1 s.
Western blot analysis of lysates from Rat testis, using PELP1 Rabbit mAb (CAB9026) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
