The PFKM Monoclonal Antibody (CAB3671) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, is highly specific and sensitive for detecting PFKM in human samples, making it ideal for Western blotting and immunohistochemistry applications.PFKM is essential for the breakdown of glucose and the production of ATP, the energy currency of cells. Dysregulation of PFKM has been linked to various metabolic disorders, including cancer and diabetes. By targeting PFKM with this monoclonal antibody, researchers can gain insights into the role of this enzyme in disease progression and potentially identify novel therapeutic targets for intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PFKM Monoclonal Antibody
SKU:
CAB3671
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0819
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
HeLa, RD, Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Cytoplasm.
Calculated MW:
85kDa
Observed MW:
85kDa
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
5213
RRID
AB_2863110
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PFKM Rabbit mAb (CAB3671) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of various lysates using PFKM Rabbit mAb (CAB3671) at 1:15000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
