The PG (Progesterone) ELISA Kit is specifically designed for the precise measurement of progesterone levels in various biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Progesterone is a key hormone that plays a vital role in the menstrual cycle, pregnancy, and overall reproductive health in both men and women. Monitoring progesterone levels is essential for assessing fertility, diagnosing hormonal imbalances, and managing certain gynecological conditions.
With its user-friendly protocol and reliable performance, the PG ELISA Kit is an indispensable tool for researchers, clinicians, and pharmaceutical companies seeking to explore the role of progesterone in reproductive health, endocrinology, and beyond. Order now to unlock valuable insights into progesterone biology and its clinical implications.
Product Name:
Pg (Progesterone) ELISA Kit
SKU:
UNES00066
Target:
Pg (Progesterone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.15 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pg. During the reaction, Pg in the sample or standard competes with a fixed amount of Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-90
83-102
84-108
Average (%)
85
90
93
1:4
Range (%)
80-95
82-95
80-112
Average (%)
89
89
92
1:8
Range (%)
89-100
81-89
86-96
Average (%)
83-90
83-102
84-108
1:16
Range (%)
85
90
93
Average (%)
80-95
82-95
80-112
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
80-94
84
EDTA plasma (n=5)
82-96
87
Cell culture media (n=5)
85-109
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.56
2.22
8.78
0.54
2.01
8.55
Standard deviation
0.05
0.14
0.53
0.05
0.14
0.53
C V (%)
8.39
6.42
5.98
9.35
7.04
6.21
Sample type &Sample volume:
serum, plasma, urine, saliva; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
