The PGAM2 Antibody (CAB7917) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has high reactivity with human samples, making it ideal for use in Western blot applications. By specifically binding to the PGAM2 protein, this antibody enables accurate detection and analysis of PGAM2 levels in various cell types.PGAM2 is essential for the conversion of 3-phosphoglycerate to 2-phosphoglycerate in the glycolytic pathway, making it a crucial enzyme in cellular energy metabolism. Dysregulation of PGAM2 has been linked to various diseases, including cancer and metabolic disorders, making it an important target for investigation in research settings.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PGAM2 Antibody
SKU:
CAB7917
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSD10, PGAMM, PGAM-M, PGAM2
Positive Sample:
22Rv1, SH-SY5Y, U-87MG, Mouse kidney, Mouse testis, Mouse skeletal muscle, Rat brain, Rat kidney, Rat skeletal muscle
Cellular Localization:
Cytosol, Extracellular Exosome, Nucleus.
Calculated MW:
29kDa
Observed MW:
29kDa
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.
Purification Method
Affinity purification
Gene ID
5224
RRID
AB_2770872
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PGAM2 Rabbit pAb (CAB7917) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunofluorescence analysis of paraffin-embedded rat heart using PGAM2 Rabbit pAb (CAB7917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
