The PGC1alpha/beta Monoclonal Antibody (CAB19674) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced specifically for use in humans, is ideal for applications such as Western blotting, immunohistochemistry, and immunofluorescence.PGC1 Alpha and Beta are key regulators of mitochondrial biogenesis and oxidative metabolism, making them essential for cells with high energy demands, such as muscle and brown adipose tissue. Dysregulation of PGC1 activity is associated with metabolic diseases, neurodegenerative disorders, and cancer, highlighting the importance of studying these proteins in various cellular contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PGC1alpha/beta Monoclonal Antibody
SKU:
CAB19674
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0153
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
Purification Method
Affinity purification
Gene ID
10891 133522
RRID
AB_2862726
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PGC1α/β Rabbit mAb (CAB19674) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
