The PHC3 Antibody (CAB6479) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, and has been validated for use in Western blot applications. By selectively binding to PHC3, this antibody enables accurate detection and analysis of the protein in various cell types, making it an essential component for studies in molecular biology and genetics.PHC3, also known as Polyhomeotic-like protein 3, is involved in chromatin remodeling and transcriptional regulation, playing a crucial role in cellular processes such as growth and differentiation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PHC3 Antibody
SKU:
CAB6479
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EDR3, HPH3, PHC3
Positive Sample:
MCF7, A-549, Mouse kidney
Cellular Localization:
Nucleus.
Calculated MW:
106kDa
Observed MW:
106kDa
Predicted to enable chromatin binding activity and histone binding activity. Predicted to be involved in negative regulation of transcription, DNA-templated. Located in nucleoplasm. Part of PRC1 complex.
Purification Method
Affinity purification
Gene ID
80012
RRID
AB_2767080
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PHC3 Rabbit pAb (CAB6479) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
