The PHF19 Antibody (CAB8065) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to PHF19, this antibody enables precise detection and analysis of the protein in a variety of cell types, making it ideal for studies in epigenetics, gene regulation, and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PHF19 Antibody
SKU:
CAB8065
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCL3, MTF2L1, TDRD19B, PHF19
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
66kDa
Observed MW:
66kDa
Enables methylated histone binding activity. Involved in positive regulation of histone H3-K27 methylation. Colocalizes with ESC/E(Z) complex.
Purification Method
Affinity purification
Gene ID
26147
RRID
AB_2770882
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PHF19 Rabbit pAb (CAB8065) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using PHF19 Rabbit pAb (CAB8065).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
