The PHF21A Antibody (CAB6330) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to PHF21A, researchers can accurately detect and analyze this protein in various cell types, providing insight into its role in molecular mechanisms related to cancer, gene expression, and epigenetic regulation.PHF21A, also known as PHD finger protein 21A, is a key player in chromatin structure organization and transcriptional regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PHF21A Antibody
SKU:
CAB6330
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BHC80, NEDMS, BM-006, IDDBCS, PHF21A
Positive Sample:
Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
75kDa
Observed MW:
75kDa
The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).
Purification Method
Affinity purification
Gene ID
51317
RRID
AB_2766933
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse liver, using PHF21A Rabbit pAb (CAB6330) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
