The PHF21B Antibody (CAB7849) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable metal ion binding activity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PHF21B Antibody
SKU:
CAB7849
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PHF4, BHC80L, PHF21B
Positive Sample:
Mouse brain, Mouse spinal cord, Rat brain
Calculated MW:
57kDa
Observed MW:
50kDa
Predicted to enable metal ion binding activity.
Purification Method
Affinity purification
Gene ID
112885
RRID
AB_2770884
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PHF21B Rabbit pAb (CAB7849) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
