The Phospho-ARHGAP35 (Y1087) Antibody (PACO02800) is a valuable tool for researchers studying the phospho-ARHGAP35 protein, which plays a crucial role in cell signaling pathways and cytoskeleton regulation. This antibody, produced in rabbits, is highly specific for the phosphorylated form of ARHGAP35 at tyrosine 1087 and is validated for use in various applications such as Western blotting.Phospho-ARHGAP35 is known to be involved in processes related to cell migration, adhesion, and polarity, making it a key player in cell physiology and cancer progression.
By targeting the phosphorylated form of ARHGAP35, researchers can gain insights into the activation and regulation of signaling cascades that affect cellular behavior.This antibody provides a reliable tool for the detection and analysis of phospho-ARHGAP35 in different cell types, making it an essential component for studies in cell biology, cancer research, and drug development. Understanding the role of phospho-ARHGAP35 in cellular processes can provide valuable knowledge for the development of targeted therapies and treatments for various diseases.
Antibody Name:
Phospho-ARHGAP35 (Y1087) Antibody
Antibody SKU:
PACO02800
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:2000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from human GRF-1 around the phosphorylation site of Y1087.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; Transcription factor; GAPs; Tumor suppressorChromosomal Location of Human Ortholog: 19q13.3Cellular Component: cytosol; nucleus; actin cytoskeletonMolecular Function: GTP binding; DNA binding; transcription corepressor activityBiological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; small GTPase mediated signal transduction; neural tube closure; forebrain development; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
