The Phospho-ARHGAP35 (Y1105) Antibody (PAC002801) is specifically designed for research involving ARHGAP35, a protein involved in cell signaling and regulation. This antibody, generated in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blotting. It binds to phosphorylated ARHGAP35 at the Y1105 site, allowing for the detection and analysis of this critical phosphorylation event in different cell types.ARHGAP35, also known as p190RhoGAP-35, plays a crucial role in regulating Rho GTPase signaling pathways, which are involved in diverse cellular processes such as cell migration, proliferation, and cytoskeletal dynamics. Phosphorylation at the Y1105 site is known to regulate the activity of ARHGAP35 and impact its interaction with other signaling proteins, making it a key player in cell signaling networks.
The Phospho-ARHGAP35 (Y1105) Antibody is a valuable tool for researchers studying cell signaling pathways, cytoskeletal dynamics, and related processes. Understanding the role of phosphorylated ARHGAP35 in these pathways is essential for elucidating its function in normal cellular physiology and disease states, including cancer and neurological disorders. This antibody allows for precise detection and quantification of phosphorylated ARHGAP35, facilitating in-depth analysis of its role in cellular signaling cascades.
Antibody Name:
Phospho-ARHGAP35 (Y1105) Antibody
Antibody SKU:
PACO02801
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from human GRF-1 around the phosphorylation site of Y1105.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; Transcription factor; GAPs; Tumor suppressorChromosomal Location of Human Ortholog: 19q13.3Cellular Component: cytosol; nucleus; actin cytoskeletonMolecular Function: GTP binding; DNA binding; transcription corepressor activityBiological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; small GTPase mediated signal transduction; neural tube closure; forebrain development; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
