The Phospho-Bad-S112 Monoclonal Antibody (CABP1010) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, and has been validated for use in Western blot applications.BAD is a pro-apoptotic protein that plays a key role in the intrinsic pathway of apoptosis, regulating cell survival and death. Phosphorylation of BAD at serine 112 is known to promote cell survival by preventing its interaction with anti-apoptotic proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-Bad-S112 Monoclonal Antibody
SKU:
CABP1010
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1555
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BBC2, BCL2L8, Phospho-Bad-S112
Positive Sample:
NIH/3T3 treated with PMA, NIH/3T3 treated with Forskolin
Cellular Localization:
Cytoplasm, Mitochondrion Outer Membrane.
Calculated MW:
18kDa
Observed MW:
23kDa
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Purification Method
Affinity purification
Gene ID
572
RRID
AB_2863899
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (CABP1010) at 1:1000 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
